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Merck
CN

C7988

Anti-Cre antibody, Mouse monoclonal

clone 7-23, purified from hybridoma cell culture

Synonym(s):

Mouse Anti-Recombinase cre

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
MFCD02262736

Key Documents

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Product Name

Anti-Cre antibody, Mouse monoclonal, clone 7-23, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

7-23, monoclonal

form

buffered aqueous solution

mol wt

antigen ~38 kDa

concentration

~2 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using recombinant Cre recombinase

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Cre (mouse IgG1 isotype) is derived from the 7-23 hybridoma produced by the fusion of mouse myeloma cells (X63-Ag8.653) and splenocytes from BALB/c mice immunized with Cre protein. Cre recombinase is a member of the integrase family of proteins that are site-specific recombinases.The protein is expressed in P1 bacteriophage.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Cre antibody ,Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunohistochemistry
  • immunocytochemistry
  • flow cytometry
  • immunostaining

Biochem/physiol Actions

Cre recombinase enables DNA recombination between two recognition sites called loxP. Each one of these sites (34 bp long) contain two 13 bp palindromic flanking sequences and in between them is a core spacer sequence 8 bp long. The recombination event takes place within the spacer area of the two loxP sites. Cre/loxP system manipulates genes in mammalian systems by making deletions, gene replacements, insertions (knockin), conditional gene targeting (knockout), and point mutations. Monoclonal antibodies to the Cre protein are an important tool for the identification of Cre recombinase in different mammalian systems.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution should be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000408648
0000351500
0000408648
0000351500
0000166652

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Susana Martin-Ortigosa et al.
Methods in molecular biology (Clifton, N.J.), 1642, 169-180 (2017-08-18)
We describe a non-DNA-based system for delivering Cre recombinase protein into maize tissue using gold-plated mesoporous silica nanoparticle (Au-MSN). Cre protein is first loaded into the pores of Au-MSNs and then delivered using the biolistic method to immature embryos of
Dopamine receptor D2, but not D1, mediates descending dopaminergic pathway-produced analgesic effect in a trigeminal neuropathic pain mouse model
Liu S, et al.
Pain, 160(2), 334-334 (2019)
Directional resolution of synthetic holliday structures by the Cre recombinase
Lee L and Sadowski PD
Test, 276(33), 31092-31098 (2001)
Fernando H Biase et al.
iScience, 7, 16-29 (2018-09-30)
We developed the Rainbow-seq technology to trace cell division history and reveal single-cell transcriptomes. With distinct fluorescent protein genes as lineage markers, Rainbow-seq enables each single-cell RNA sequencing (RNA-seq) experiment to simultaneously decode the lineage marker genes and read single-cell
A Model for Epigenetic Inhibition via Transvection in the Mouse
Rodriguez JD, et al.
Genetics, 207(1), 129-138 (2017)
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