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Merck
CN

C8210

CHES

BioXtra, ≥99.0% (titration)

Synonym(s):

2-(Cyclohexylamino)ethanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
103-47-9
Molecular Weight:
207.29
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24893031
EC Number:
203-115-6
Beilstein/REAXYS Number:
2967601
MDL number:
MFCD00003835

Key Documents

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Product Name

CHES, BioXtra, ≥99.0% (titration)

InChI key

MKWKNSIESPFAQN-UHFFFAOYSA-N

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

SMILES string

OS(=O)(=O)CCNC1CCCCC1

product line

BioXtra

assay

≥99.0% (titration)

form

powder

impurities

≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

3.0-5.0 (0.5 M in H2O)

useful pH range

8.6-10.0

pKa (25 °C)

9.3

solubility

H2O: 0.5 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.01%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.05 at 280 in H2O at 0.5 M
≤0.08 at 260 in H2O at 0.5 M

application(s)

diagnostic assay manufacturing

Quality Level

200

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Application

CHES may be used:
  • for pH adjustments and as a assay buffer in catechol oxidase activity measurements(32)
  • as a buffer in electron paramagnetic resonance (EPR) spectroscopy and kinetic measurements(33)
  • as a buffer for soil solution equivalent (SSE) yeast dextrose medium for screening pH optima for growth of Aridibacter nitratireducens A24_SHP-5_238T(34)

General description

CHES (2-(Cyclohexylamino) ethane sulfonic acid) is a derivative of amino acid taurine. It serves as a standard physiological buffer in the pH range of 8.5-9.5.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000508830
0000508830
0000501448
0000501448
0000497708

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Buffers for the physiological pH range: Acidic dissociation constants of zwitterionic compounds (ACES and CHES) in water from 5 to 55? C
Roy RN, et al.
Journal of Chemical and Engineering Data, 42(1), 41-44 (1997)
Jun Yang et al.
Langmuir : the ACS journal of surfaces and colloids, 24(18), 10265-10272 (2008-08-20)
A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to
R C Bray et al.
Biochemistry, 39(37), 11258-11269 (2000-09-14)
Much is unknown concerning the role of thiolate ligands of molybdenum in molybdopterin enzymes. It has been suggested that thiolate dissociation from molybdenum is part of the catalytic mechanism of bis-molybdopterin enzymes of the dimethyl sulfoxide reductase (DMSOR) family. For
Hao Zhong et al.
The Analyst, 136(21), 4486-4491 (2011-09-29)
On-line concentration via Electrokinetic Supercharging (EKS) was used to enhance the sensitivity of the capillary electrophoretic separation of the four flavonoids naringenin, hesperetin, naringin and hesperidin. Separation conditions, including the background electrolyte pH and concentration, the length and choice of
A D Carraway et al.
Journal of inorganic biochemistry, 60(4), 267-276 (1995-12-01)
Electron paramagnetic resonance spectra of a number of ferric heme peptide derivatives, in aqueous-detergent and various aqueous-alcohol solvent mixtures, have been obtained using samples in the concentration range 0.1-1.0 mM. Some of these were clearly monomeric, homogeneous, mixed-ligand adducts, entirely
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