C8413
Chloramphenicol Acetyltransferase from Escherichia coli
buffered aqueous glycerol solution
Synonym(s):
Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT
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About This Item
Specific activity:
50,000-150,000 units/mg protein
form
buffered aqueous glycerol solution
specific activity
50,000-150,000 units/mg protein
mol wt
75 kDa (three identical subunits)
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli ... cat(2847485)
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Application
Chloramphenicol acetyltransferase from Escherichia coli has been used in a study to assess the construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production. Chloramphenicol acetyltransferase from Escherichia coli has also been used in a study to investigate site-directed mutagenesis and promoter functional analysis of the RM07 DNA fragment from Halobacterium halobium.
The enzyme has been used in chloramphenicol acetyltransferase assay to optimize the transfection of plasmid DNA into primary cultures of adult mouse keratinocytes. It has also been used to assess the acetyl-CoA carboxylase-carboxyltransferase (ACC-CT) domain activity. This has been done using a coupled-two phase system measuring the selective partition of [14C]acetylchloramphenicol into an organic layer.
Biochem/physiol Actions
The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.
Physical form
Clear, colorless solution in 50% glycerol containing 5 mM Tris-HCl, pH 7.8, and 0.5 mM 2-mercaptoethanol
Other Notes
One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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N A Betz et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 28A(3 Pt 1), 188-192 (1992-03-01)
An efficient and reproducible technique for the transfection of primary cultures of adult mouse keratinocytes has been developed. The procedure involves culturing the primary adult mouse epidermal cells at 32 degrees C in an enriched media until they reach 70
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Dian-Li Zhao et al.
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Pseudoalteromonas is an important genus widespread in marine environment, and a lot of psychrophilic Pseudoalteromonas strains thrive in deep sea and polar sea. By now, there are only a few genetic systems for Pseudoalteromonas reported and no commercial Pseudoalteromonas genetic
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Journal of applied toxicology : JAT, 32(10), 783-789 (2011-04-19)
Reporter gene assays are commonly used in applied toxicology to measure the transcription of genes involved in toxic responses. In these reporter gene assays, transgenic cells are used, which contain a promoter-operator region of a gene of interest fused to
Zhiyang Li et al.
Journal of nanoscience and nanotechnology, 11(2), 1074-1078 (2011-04-02)
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