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C8667

Sigma-Aldrich

Cholesterol

Sigma Grade, ≥99%

Synonym(s):

3β-Hydroxy-5-cholestene, 5-Cholesten-3β-ol

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10 EA
CN¥7,971.28

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10 EA
CN¥7,971.28

About This Item

Empirical Formula (Hill Notation):
C27H46O
CAS Number:
Molecular Weight:
386.65
Beilstein:
1915888
EC Number:
MDL number:
UNSPSC Code:
12352111
eCl@ss:
39023139
PubChem Substance ID:
NACRES:
NA.77

CN¥7,971.28


Estimated to ship onOctober 09, 2025Details


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biological source

sheep wool

Quality Level

grade

Sigma Grade

Assay

≥99%

form

powder

bp

360 °C (lit.)

mp

147-149 °C (lit.)

solubility

water: soluble 0.00003 g/L at 20 °C

density

1.067 g/mL at 25 °C (lit.)

shipped in

ambient

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This Item
82485P603182526
recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

assay

≥95% (HPLC)

assay

≥90% protein basis

assay

-

assay

-

form

aqueous solution

form

fibers

form

essentially salt-free, lyophilized powder

form

powder

mol wt

45,000

mol wt

Mr ~41000

mol wt

-

mol wt

-

UniProt accession no.

P02976

UniProt accession no.

P02976

UniProt accession no.

P02976

UniProt accession no.

P02976

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-

Application

Cholesterol was used to prepare immune-stimulating complexes to enhance cellular and humoral responses.[1] It was incorporated in animal feed to study the effect of cholesterol-rich diet.[2][3]
Standard for chromatography

Biochem/physiol Actions

Cholesterol is a lipid that makes up about 20-25% of the structural components of the cell membranes. It determines the fluidity and permeability of the membrane, making it permeable to water but not to ions and protons. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane.[4] The major sites of cholesterol synthesis are small intestine and liver.[5]
Major component of all biological membranes; ~25% of total brain lipid is cholesterol.

Preparation Note

Cholesterol monohydrate yields a clear, colorless to faint yellow solution in hot alcohol. It is also soluble in acetone, dioxane, ethyl acetate, benzene, petroleum ether, oils, fats, and in aqueous solutions of bile salts. Solutions should be protected from light.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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