C9017
Concanavalin A from Canavalia ensiformis (Jack bean)
Sepharose™ conjugate, buffered aqueous suspension
Synonym(s):
ConA
biological source
Canavalia ensiformis
Quality Level
conjugate
Sepharose™ conjugate
form
buffered aqueous suspension
contains
20% ethanol as preservative
extent of labeling
10-16 mg per mL
matrix
Sepharose 4B
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥25 mg/mL binding capacity (thyroglobulin)
storage temp.
2-8°C
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Application
Used for the purification of glycoproteins containing mannose.
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Physical form
Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl2 , MgCl2, and MnCl2
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
102.2 °F
Flash Point(C)
39 °C
Regulatory Information
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Jae-Uoong Shim et al.
Inflammation, 32(4), 211-217 (2009-05-06)
Cudrania tricuspidata is one of the most omnipresent traditional herbal drugs for anti-inflammation and anti-tumor. The purpose of the present study was to determine whether the CTB glycoprotein regulates the inflammatory reaction stimulated by bisphenol A (BPA) in human mast
Jin Lee et al.
Journal of cellular biochemistry, 112(2), 694-703 (2011-01-27)
Cadmium is one of the inflammation-related xenobiotics and has been regarded as a potent carcinogen. Gardenia jasminoides Ellis (GJE) has been used to cure inflammation in Korean folk medicine for a long time. The purpose of present study is the
Robert J Huber et al.
Matrix biology : journal of the International Society for Matrix Biology, 31(4), 271-280 (2012-03-07)
CyrA, an extracellular matrix (slime sheath), calmodulin (CaM)-binding protein in Dictyostelium discoideum, possesses four tandem EGF-like repeats in its C-terminus and is proteolytically cleaved during asexual development. A previous study reported the expression and localization of CyrA cleavage products CyrA-C45
Phil-Sun Oh et al.
Journal of cellular biochemistry, 109(1), 124-131 (2009-11-04)
This study investigated the inhibitory effect of a glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) on di(2-ethylhexyl) phthalate (DEHP)-induced mast cell degranulation and related signaling cascade in RBL-2H3 cells. This experiment evaluated the intracellular Ca(2+) level, and the activities
Ji-Yun Kim et al.
Chinese journal of natural medicines, 18(10), 738-748 (2020-10-12)
Dioscorea batatas Decne (DBD) has been used to heal various illnesses of the kidney and intestine as an herbal medicine in Asia. As a source of therapeutic agents, many glycoproteins have been isolated from mushrooms and plants, but the functional
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