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C9281

Sigma-Aldrich

Cholesterol Esterase from Pseudomonas fluorescens

lyophilized powder, ≥10,000 units/g protein

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Synonym(s):
CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase
CAS Number:
9026-00-0
Enzyme Commission number:
3.1.1.13 (BRENDA, IUBMB)
EC Number:
232-808-6
MDL number:
MFCD00071052
NACRES:
NA.54

biological source

Pseudomonas fluorescens

Quality Level

200

Assay

10-30% (TCA-Biuret)

form

lyophilized powder

specific activity

≥10,000 units/g protein

mol wt

~129 kDa

composition

Protein, ~20%

storage condition

under inert gas (argon)

technique(s)

cell based assay: suitable

color

tan to brown

pH

7-9

solubility

0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL

storage temp.

−20°C

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General description

Research area: Cell Signaling

Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).

Application

Cholesterol esterase from Pseudomonas fluorescens has been used in:
  • in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
  • a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
  • an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
  • for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
  • to hydrolyze native cholesterol ester (CE) during ​filipin staining for detection of CE within the retinal frozen sections
This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..

Other Notes

Contains potassium phosphate and TRITON® X-100.

Unit Definition

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Analysis Note

Protein determined by biuret.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Nanomedicine
Viswanathan S, et al.
Methods in Enzymology, 693, 56-66 (2012)
Carolina Espinosa Álvarez et al.
Molecules (Basel, Switzerland), 25(24) (2020-12-30)
Haematococcus pluvialis is the largest producer of natural astaxanthin in the world. Astaxanthin is a bioactive compound used in food, feed, nutraceutics, and cosmetics. In this study, astaxanthin extraction from H. pluvialis by supercritical fluid extraction was evaluated. The effects
A colorimetric CMOS-based platform for rapid total serum cholesterol quantification
Al-Rawhani MA, et al.
IEEE Sensors Journal, 17, 240-247 (2016)
Syed A H Zaidi et al.
Journal of neuroinflammation, 20(1), 14-14 (2023-01-24)
We have investigated the efficacy of a new strategy to limit pathological retinal neovascularization (RNV) during ischemic retinopathy by targeting the cholesterol metabolizing enzyme acyl-coenzyme A: cholesterol transferase 1 (ACAT1). Dyslipidemia and cholesterol accumulation have been strongly implicated in promoting
Jounghyun H Lee et al.
Advanced biology, 5(4), e2000428-e2000428 (2021-04-15)
Atherosclerosis begins with the accumulation of cholesterol-carrying lipoproteins on blood vessel walls and progresses to endothelial cell dysfunction, monocyte adhesion, and foam cell formation. Endothelialized tissue-engineered blood vessels (TEBVs) have previously been fabricated to recapitulate artery functionalities, including vasoconstriction, vasodilation
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Cholesterol Esterification

Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.

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