C9658
Carboxypeptidase G from Pseudomonas sp.
lyophilized powder, ≥3 units/mg protein
Synonym(s):
γ-Glutamyl hydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-978-1
MDL number:
Specific activity:
≥3 units/mg protein
form
lyophilized powder
Quality Segment
specific activity
≥3 units/mg protein
mol wt
homodimer ~90 kDa
composition
Protein, ~70% biuret
solubility
H2O: soluble 0.5 mg/mL
storage temp.
−20°C
Application
Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.
Biochem/physiol Actions
Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.
Physical form
Contains sodium acetate salt.
Preparation Note
Chromatographically purified
Solutions should be prepared fresh prior to use.
Other Notes
One unit will hydrolyze 1.0 μmole of L -glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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