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C9999

Sigma-Aldrich

Citrate Buffer, pH 6.0, 10×, Antigen Retriever

for immunohistochemistry

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Synonym(s):
1,2,3-Propanetricarboxylic acid, 2-hydroxy-, mixt. with sodium hydroxide (Na(OH)) and sodium 2-hydroxy-1,2,3-propanetricarboxylate
NACRES:
NA.47

Quality Level

form

liquid

pH

6.0

application(s)

hematology
histology

storage temp.

2-8°C

Application

Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

Preparation Note

Dilute the Citrate buffer, pH 6.0, 10x, Antigen Retriever 10-fold with water to prepare a 1x Working Solution, e.g., dilute 10 mL of 10x concentrate with 90 mL of water.

Storage and Stability

Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.

Other Notes

Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.

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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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