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Merck
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MRC-5 PD 19 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5072101, human lung, Fibroblast-like

Synonym(s):

MRC-V Cells, MRC5 Cells, Medical Research Council Cell Strain-5

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

human lung

description

Human fetal lung

packaging

tube of 5 μg 05072101-DNA-5UG
pkg of vial of cells 05072101-1VL

growth mode

Adherent

karyotype

2n=46, diploid

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

It has been used as a cell substrate to study the production of recombinant MVA (modified vaccinia virus Ankara).

Biochem/physiol Actions

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.
Human foetal lung
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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