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SPC212

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Synonym(s):

SPC 212, SPC-212

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About This Item

UNSPSC Code:
41106514
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biological source

human lung

packaging

tube of 5 μg 11120717-DNA-5UG
pkg of vial of cells 11120717-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epitheloid/mesenchymal

receptors

Mesothelial-membrane antigen ME1

relevant disease(s)

cancer

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Biochem/physiol Actions

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
SPC212 was derived from a biphasic tumour of the pleural cavity of a 47-year old female patient, prior to treatment, with a known history of exposure to asbestos.
STR-PCR Data:

Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16

Preparation Note

DMEM:F12 (1:1) (D8062) + L-Glutamine (G7513) + 15% FBS / FCS (F2442).
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm2.

Other Notes

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