biological source
human lung
packaging
tube of 5 μg 11120717-DNA-5UG, pkg of vial of cells 11120717-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epitheloid/mesenchymal
receptors
Mesothelial-membrane antigen ME1
relevant disease(s)
cancer
Biochem/physiol Actions
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
SPC212 was derived from a biphasic tumour of the pleural cavity of a 47-year old female patient, prior to treatment, with a known history of exposure to asbestos.
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16
Preparation Note
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm2.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.