biological source
human blood
packaging
tube of 5 μg 88022014-DNA-5UG
pkg of vial of cells 88022014-1VL
ethnicity
North American Hispanic
gender
female
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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General description
Homozygous: Yes
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 2601
Cw: 701
B: 801
HLA DRA: 102
HLA DRB1: 1501
HLA DRB5: 101
HLA DRB6: 201
HLA DQAP: 1.2
HLA DQA1: 1021
HLA DQB1: 602
HLA DPA1: 1
HLA DPB1: 401
Cw: 701
B: 801
HLA DRA: 102
HLA DRB1: 1501
HLA DRB5: 101
HLA DRB6: 201
HLA DQAP: 1.2
HLA DQA1: 1021
HLA DQB1: 602
HLA DPA1: 1
HLA DPB1: 401
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
HLA A: 26
HLA A ief: 26.1
HLA C: 7
HLA B: 8
HLA B ief: 8
Bw4/bw6: 6
HLA Dw: 2
HLA DR: 15
HLA DR51: 51
HLA DQ: 6
HLA DP: 4
HLA A ief: 26.1
HLA C: 7
HLA B: 8
HLA B ief: 8
Bw4/bw6: 6
HLA Dw: 2
HLA DR: 15
HLA DR51: 51
HLA DQ: 6
HLA DP: 4
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
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