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About This Item
UNSPSC Code:
12352200
Biological source:
human colon
Growth mode:
Adherent
Karyotype:
modal No 46
Morphology:
Fibroblast
Product Name
CCD-18Co, 90070503
biological source
human colon
growth mode
Adherent
karyotype
modal No 46
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Aging studies
Biochem/physiol Actions
Derived from biopsy colon tissue of a black female infant (2.5 months old). CCD 18Lu (90112706) and CCD-18Co (90070503) were derived from the lung and colon respectively from the same patient; this has been confirmed by STR profiling at ECACC.
Human colon fibroblast, normal, ethnicity: Black
STR-PCR Data: Amelogenin: X
CSF1PO: 8
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 8
THO1: 6,7
TPOX: 8,11
vWA: 15,17
CSF1PO: 8
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 8
THO1: 6,7
TPOX: 8,11
vWA: 15,17
Preparation Note
EMEM (EBSS) + 2mM glutamine + 1% NEAA + 10% FBS
Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Regulatory Information
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