STE-137

Isolation of viruses from clinical samples, virus propagation

Fish steelhead trout embryo

STE-137 was initiated in 1963 from pooled steelhead embryos (Oncorynchus mykiss). This heteroploid cell line, also known as steelhead embryo-137, replicates and detects common salmonid viruses (infectious pancreatic necrotic virus (IPNV), infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV)). The growth temperature ranges from 15-21°C with a routine propagation at 18°C.

MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 18°C. Population doubling can take up to 72 hours. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr J L Fryer, Department of Microbiology, Oregon State University, Corvallis