STE-137
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
fish embryo
packaging
tube of 5 μg 95122020-DNA-5UG
pkg of vial of cells 95122020-1VL
growth mode
Adherent
karyotype
Modal no. 58
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Isolation of viruses from clinical samples, virus propagation
Biochem/physiol Actions
Fish steelhead trout embryo
STE-137 was initiated in 1963 from pooled steelhead embryos (Oncorynchus mykiss). This heteroploid cell line, also known as steelhead embryo-137, replicates and detects common salmonid viruses (infectious pancreatic necrotic virus (IPNV), infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV)). The growth temperature ranges from 15-21°C with a routine propagation at 18°C.
Preparation Note
MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 18°C. Population doubling can take up to 72 hours. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Dr J L Fryer, Department of Microbiology, Oregon State University, Corvallis
Regulatory Information
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