CDA00011E
US-2019-nCoV SC2-HEX for detection of SARS-CoV-2 RNA
form
solution (in 10 mM Tris, 1 mM EDTA)
packaging
pkg of 1 vial
pH range
7.5-8.5
shipped in
ambient
storage temp.
−20°C
General description
The US-2019-nCoV SC2 Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2. The product is primers and probes, the sequences of which were designed and made publicly available by the United States Centers for Disease Control and Prevention (U.S. CDC) for use in its multiplex respiratory virus panel.
For additional details, please visit SigmaAldrich.com/ncov.
For additional details, please visit SigmaAldrich.com/ncov.
Features and Benefits
The forward and reverse primers in each tube come with the following features:
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- No modifications
- Desalt purified
- 16 nmol each
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- 6-FAM™ reporter
- BXQ (Block All Quencher—SigmaAldrich.com/bxq)
- HPLC purified
- 4nmol
Other Notes
The product includes 1 tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene. Assay included is N (3′ end), also known as SC2.
The forward primer, reverse primer, and probe are at final concentrations of 16, 16, and 4 μM, respectively.
SC2 Assay:
Forward Primer CTGCAGATTTGGATGATTTCTCC
Reverse Primer CCTTGTGTGGTCTGCATGAGTTTAG
Dual-Labeled Probe ATTGCAACAATCCATGAGCAGTGCTGACTC
The forward primer, reverse primer, and probe are at final concentrations of 16, 16, and 4 μM, respectively.
SC2 Assay:
Forward Primer CTGCAGATTTGGATGATTTCTCC
Reverse Primer CCTTGTGTGGTCTGCATGAGTTTAG
Dual-Labeled Probe ATTGCAACAATCCATGAGCAGTGCTGACTC
Legal Information
FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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