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CMC0014

Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(s):

Competent E. coli cells

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100 EA
¥589.22

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100 EA
¥589.22

About This Item

UNSPSC Code:
41106202
NACRES:
NA.85

¥589.22


Please contact Customer Service for Availability

Product Name

BL21(DE3) Chemically Competent Cells, for protein expression

biological source

Escherichia coli

grade

Molecular Biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

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This Item
Z683604Z683558Z683566
feature

graduated 0.5 mL

feature

graduated 0.5 mL

feature

graduated 0.1 mL

feature

graduated 0.1 mL

packaging

pkg of 100 ea bulk packed

packaging

pkg of 100 ea bulk packed

packaging

pkg of 100 ea bulk packed

packaging

pkg of 100 ea bulk packed

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

capacity

10 mL

capacity

10 mL

capacity

3 mL

capacity

3 mL

General description

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.[1]
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.[2]
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.[3][1]

Features and Benefits

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Components

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

Storage Class Code

10 - Combustible liquids


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