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Merck
CN

CS0610

Sigma-Aldrich

Casein Kinase II Assay Kit

sufficient for 70 reactions

Synonym(s):

CKII Assay Kit

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About This Item

UNSPSC Code:
12352200
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usage

sufficient for 70 reactions

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

The Casein Kinase II Assay Kit measures the CKII enzyme activity by in vitro phosphorylation of the Casein Kinase II Substrate. This substrate is a peptide that is selective for CK II with no activity with CK I.
The kit also contains a CK II specific inhibitor to ensure the specificity of the CK activity, and purified CK II for use as a positive control.

Biochem/physiol Actions

Casein Kinase type II (CK II) is a tetrameric enzyme of 130-150 kDa with α2β2 structure. The α subunit is catalytic and the β subunit is thought to have regulatory properties. CK II is expressed in the nucleus as well as in the cytoplasm and mitochondria. It has been implicated in a variety of cellular processes and is important for signaling pathways controlling growth division including mitosis, cellular transformation, and differentiation of cells

Kit Components Only

Product No.
Description

  • Assay Buffer for Casein Kinase Activity 1.5 mL

  • ATP Solution .5 mL

  • Casein Kinase II Substrate 1 mg

  • Casein Kinase II from Rat Liver 1 U

  • Heparin (CKII Inhibitor) 1 mL

  • Enzyme Dilution Buffer 1.7 mL

  • P81 Cellulose Phosphate Squares 10 ea

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Find documentation for the products that you have recently purchased in the Document Library.

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Casein kinase 2: an 'eminence grise' in cellular regulation?
L A Pinna
Biochimica et biophysica acta, 1054(3), 267-284 (1990-09-24)

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