CS0770
NDPase (GDPase, UDPase, ADPase) Assay Kit
sufficient for 300 assays (96-well plates)
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About This Item
UNSPSC Code:
12352200
usage
sufficient for 300 assays (96-well plates)
shipped in
dry ice
storage temp.
−20°C
Application
A simple and reliable method for measuring NDPase activity in sub-cellular fractions based on the detection of the inorganic phosphate released from NDP by the appropriate NDPase. The color formation, measured at 700 nm in the presence of molybdate, is proportional to the amount of the inorganic phosphate released by enzymes.
Biochem/physiol Actions
GDPases/UDPases (NDPases) in yeasts are located exclusively in the Golgi complex and are a convenient marker for Golgi detection. NDPs are degraded to NMPs by nucleoside diphosphatase (GDPase/UDPase). The NMPs are then used in antiport transport of new nucleotide sugars into the organelle lumen. ADPases are known to be expressed on the cell plasma membrane (endothelial cells and vascular precursors) where they participate in nucleotide metabolism.
Analysis Note
The kit was tested on rat tissue extracts (liver, kidney, spleen, and heart) and on a yeast (S. cerevisae) extract. It was also tested with microsomal fractions prepared from rat tissues and cell lines.
Other Notes
1 unit of NDPase releases 1 μmole of inorganic phosphate per minute at 25 °C under conditions specified by the assay.
Kit Components Only
Product No.
Description
- ADPase Assay Reagent 10 mL
- GDPase Assay Reagent 10 mL
- UDPase Assay Reagent 10 mL
- Ascorbic Acid Solution 1 mL
- Molybdenum Color Reagent 60 mL
- Sodium dodecyl sulfate Solution 10% 5 mL
- Phosphorus standard Solution 0.65 mM 1.7 mL
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N Braun et al.
The Biochemical journal, 351 Pt 3, 639-647 (2000-10-24)
We have isolated and characterized the cDNA encoding nucleoside triphosphate diphosphohydrolase 6 (NTPDase6), a novel member of the ecto-nucleoside triphosphate diphosphohydrolase family. The rat-brain-derived cDNA has an open reading frame of 1365 bp encoding a protein of 455 amino acid
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