Anti-Human CD2 (TS1/8) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

T-cell surface antigen CD2 (UniProt: P06729; also known as Erythrocyte receptor LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5, CD2) is encoded by the CD2 (also known as SRBC) gene (Gene ID: 914) in human. CD2 is a transmembrane glycoprotein of the immunoglobulin superfamily expressed on the surface of T cells, NK cells, thymocytes, and dendritic cells. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-209), a transmembrane domain (aa 210-235), and a cytoplasmic domain (aa 236-351). In its extracellular domain, it contains an Ig-like V-type domain (aa 25-128) and an Ig-Like C2 type domain (aa 129-209). CD2 serves as a receptor for the lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. The membrane-proximal domain of its extracellular region connects the adhesion (membrane-distal) domain to the cell membrane and may be involved in CD2 clustering following T cell activation. The membrane-distal domain is responsible for CD58 binding. CD2 is also involved in human T cell development and activation. It plays an important role in the formation and organization of the immunological synapse that is formed between T cells and antigen-presenting cells upon cell-cell conjugation and associated intracellular signaling. Its expression is reported to be upregulated on memory T cells as well as activated T cells. (Ref.: Binder, C., et al. (2020). Front. Immunol. 11; 1090; Moingeon, P., et al. (1989). Immunol. Rev. 111; 111-144).

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Cytotoxic T lymphocytes

Quality Control Testing:

Evaluated by Flow Cytometry in Human PBMCs.

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1027, Anti-Human CD2 (TS1/8) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® activated Phycoerythrin (PE) Dye or the equivalent amount of Phycoerythrin (PE)-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone TS1/8 is a mouse monoclonal antibody that detects human T-cell surface antigen CD2 (CD2).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.