Anti-Human CD85j/LILRB1 (GHI/75) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Leukocyte immunoglobulin-like receptor subfamily B member 1 (UniProt: Q8NHL6;also known as LIR-1;Leukocyte immunoglobulin-like receptor 1;CD85 antigen-like family member J;Immunoglobulin-like transcript 2;ILT-2;Monocyte/macrophage immunoglobulin-like receptor 7;MIR-7;CD85j) is encoded by the LILRB1 (also known as ILT2;LIR1;MIR7) gene (Gene ID: 10859) in human. CD85j is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23);which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-461);a transmembrane domain (aa 462-482);and a cytoplasmic domain (aa 483-650). Its extracellular domain contains four Ig-like C2 type domains and the cytoplasmic domain has four ITIM motifs. Its expression is observed in B cells;monocytes;and various dendritic cell (DC) subsets. CD85j serves as a receptor for class I MHC antigens. It recognizes a broad spectrum of HLA-A;HLA-B;HLA-C;HLA-G;and HLA-F all

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Hairy cell leukemia cells.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC) gated on monocytes.

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on monocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1185, Anti-Human CD85j/LILRB1 (GHI/75) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1010 Mouse IgG2b isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on monocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1185, Anti-Human CD85j/LILRB1 (GHI/75) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1010 Mouse IgG2b isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone GHI/75 is a mouse monoclonal antibody that detects CD85j. It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.