Anti-Human CD272/BTLA (MIH26) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

B- and T-lymphocyte attenuator (UniProt: Q7Z6A9;also known as B- and T-lymphocyte-associated protein;CD272) is encoded by the BTLA gene (Gene ID: 151888) in human. CD272 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-30);which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 31-157);a transmembrane domain (aa 58-178);and a cytoplasmic domain (aa 179-289). The cytoplasmic region contains three tyrosine residues;which comprise a Grb-2 binding site;an immunoreceptor tyrosine-based inhibitory motif (ITIM);and an immunoreceptor tyrosine-based switch motif (ITSM). It exhibits sequence homology to PD-1 and CTLA4 and its expression is observed on T and B lymphocytes and other cells of hemopoietic lineages. CD272 serves as an inhibitory receptor on lymphocytes that negatively regulate antigen receptor signaling via SHP-1 and SHP-2. It may also interact in cis or in trans forms with TNFRSF14. In

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L cells transfected with human CD272.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC), gated on lymphocytes.

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes was performed with APC conjugated anti-human CD19 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1190, Anti-Human CD272/BTLA (MIH26) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1016 Mouse IgG2a isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye. CD19 expressing cells also express CD272.

Tested Applications

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes was performed with PE conjugated anti-human CD19 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1190, Anti-Human CD272/BTLA (MIH26) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1016 Mouse IgG2a isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye. CD19 expressing cells also express CD272.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone MIH26 is a mouse monoclonal antibody that detects CD272. It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.