ColorWheel^® Antibody-Ready FITC Dye

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that is one of the simplest and most commonly used reagents for antibody and protein labeling applications. It is suitable for use in fluorescence microscopy, Flow Cytometry, and other immunofluorescence-based assays. Its NHS ester form reacts efficiently with primary amino groups (-NH2) in neutral to slightly alkaline (pH 7 to 9) buffers to form stable amide bonds. It displays excitation max at 494 nm and emission at 518 nm and has an extinction coefficient of 70,000 M-1 cm-1.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Quality Control Testing

Flow Cytometry: Excitation: 491 nm, Emission: 516 nm

CWDS-FITC-25UL Set ComponentsCWDS-FITC-25UL - ColorWheel^® Antibody-Ready FITC DyeLyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.CWD-FINISH-25UL - ColorWheel Finishing BufferLyophilized from PBS. Contains no biocide or preservatives, such as azide.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.