CYP1
CelLytic™ Y Plus Kit
For enzymatic yeast cell lysis
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About This Item
NACRES:
NA.56
UNSPSC Code:
12352200
shipped in
dry ice
storage temp.
−20°C
Application
The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immunoreactivity and biological activity.
The cell extract is compatible with:
The cell extract is compatible with:
- Reporter gene expression assays (e.g., β-galactosidase, alkaline-phosphatase)
- Immunoassays (Western blots, immunoprecipitation)
- Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins)
- DNA-protein interaction assay (e.g., gel-shift)
- Coomassie and silver staining
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- Reaction Buffer 120 mL
- 1 M DTT 5 mL
- Lyticase 25000 U
- Extraction buffer 60 mL
- Triton™ X-100 10 mL
- Protease inhibitor cocktail 2 mL
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
Regulatory Information
监管及禁止进口产品
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Articles
CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.
我们的酵母细胞裂解试剂具有通用性、无磷酸盐、非变性等特点。CelLytic Y细胞裂解试剂可作为酵母细胞裂解和蛋白溶解的有效方法。CelLytic Y酵母酶解试剂盒为从酿酒酵母、巴斯德毕赤酵母和粟酒裂殖酵母酵母菌株中高效形成原生质球和提取蛋白提供了一种便捷的方法。
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Instructions
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