D0188
Anti-Deoxyribonuclease I (DNase I) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
UNSPSC Code:
12352203
MDL number:
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 33 kDa
species reactivity
bovine
concentration
1.0 mg/mL
technique(s)
microarray: suitable, western blot: 1 μg/mL using bovine pancreatic DNase I
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
bovine ... DNASE1(282217)
General description
The enzyme is Ca2+ and Mg2+ dependent. It is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum.
Immunogen
bovine pancreatic deoxyribonuclease I (DNase I).
Application
Anti-Deoxyribonuclease I (DNase I) antibody produced in rabbit is suitable for microarray and western blotting at a working concentration of 1 μg/mL using bovine pancreatic DNase I.
Biochem/physiol Actions
DNase I is an endonuclease that hydrolyzes double-stranded or single-stranded DNA preferentially at sites adjacent to pyrimidine nucleotides to produce a complex mixture of 5′-phosphate mononucleotides and oligonucleotides.
Physical form
Solution in phosphate buffered saline, pH 7.2, containing 0.01% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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S Shak et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(23), 9188-9192 (1990-12-01)
Respiratory distress and progressive lung destruction in cystic fibrosis can be attributed to bacterial persistence and the accumulation of viscous purulent secretions in the airways. More than 30 yr ago it was suggested that the large amounts of DNA in
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