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Merck
CN

D0758

2′-Deoxyinosine 5′-triphosphate trisodium salt

synthetic, 95-97%

Synonym(s):

dITP-Na3

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About This Item

Empirical Formula (Hill Notation):
C10H12N4Na3O13P3
CAS Number:
95648-77-4
Molecular Weight:
558.11
UNSPSC Code:
41106305
PubChem Substance ID:
24893291
EC Number:
306-018-8
MDL number:
MFCD00167408

Key Documents

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InChI key

FHYSVBOUQFJKCI-PWDLANNDSA-K

InChI

1S/C10H15N4O13P3.3Na/c15-5-1-7(14-4-13-8-9(14)11-3-12-10(8)16)25-6(5)2-24-29(20,21)27-30(22,23)26-28(17,18)19;;;/h3-7,15H,1-2H2,(H,20,21)(H,22,23)(H,11,12,16)(H2,17,18,19);;;/q;3*+1/p-3/t5-,6+,7+;;;/m0.../s1

SMILES string

[Na+].[Na+].[Na+].O[C@H]1C[C@@H](O[C@@H]1COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)n2cnc3C(=O)NC=Nc23

biological source

synthetic

assay

95-97%

shipped in

dry ice

storage temp.

−20°C

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Application

2′-Deoxyinosine 5-triphosphate (dITP) may be formed by nitrosative deamination processes such as those that deaminate adenosine in DNA and RNA. dITP may be useful in studies of this process.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
104H7859
071H7860
070K7012
047H7832
115H7816

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Gel compressions and artifact banding can be resolved in the same DNA sequence reaction.
K W McCrea et al.
BioTechniques, 15(5), 843-844 (1993-11-01)
ITPA protein, an enzyme that eliminates deaminated purine nucleoside triphosphates in cells.
Sakumi K, Abolhassani N, et al.
Mutation Research, 703, 4-50 (2010)
T Auer et al.
Nucleic acids research, 24(24), 5021-5025 (1996-12-15)
The ability to selectively amplify RNA in the presence of genomic DNA of analogous sequence is cumbersome and requires implementation of critical controls for genes lacking introns. The convenient approaches of either designing oligonucleotide primers at the splice junction or
Random mutagenesis by using mixtures of dNTP and dITP in PCR.
O P Kuipers
Methods in molecular biology (Clifton, N.J.), 57, 351-356 (1996-01-01)
Zun Wang et al.
Molecular biotechnology, 53(1), 49-54 (2012-02-22)
An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the
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