D1918
(S)-Diacetyl Reductase from baker′s yeast (S. cerevisiae)
lyophilized powder, 0.25-0.5 unit/mg solid
form
lyophilized powder
specific activity
0.25-0.5 unit/mg solid
storage temp.
2-8°C
Biochem/physiol Actions
Enantioselectively and regioselectively reduces diketones, oxoaldehydes, methyl ketones, oxoesters and cyclohexanediones to their S-configuration hydroxy-derivatives.
Physical form
Lyophilized powder containing Tris and DTT
Other Notes
One unit will convert 1 μmole of diacetyl and NADPH to (S)-acetoin and NADP per min at pH 6.9 at 25°C. Enantioselectively and regioselectively reduces diketones, oxoaldehydes, methyl ketones, oxoesters and cyclohexanediones to their S-configuration
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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J Heidlas et al.
European journal of biochemistry, 188(1), 165-174 (1990-02-22)
The NADPH-linked diacetyl reductase system from the cytosolic fraction of Saccharomyces cerevisiae has been resolved into two oxidoreductases catalyzing irreversibly the enantioselective reduction of diacetyl (2,3-butanedione) to (S)- and (R)-acetoin (3-hydroxy-2-butanone) [so-called (S)- and (R)-diacetyl reductases] (EC 1.1.1.5) which have
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