D2376
Deoxyribonucleic acid methyl green
DNAse substrate
Synonym(s):
Deoxyribonucleic acid–methyl green complex, DNA-Methyl Green
form
solid
storage temp.
−20°C
General description
Methyl green is a widely used nuclei counterstain for histochemical methods. Methyl green binds to the DNA major groove and is used for tracking triplex formation. The positive charge on the aniline rings in the methyl green enables its strong binding to DNA. Methyl green aids clear visualization in confocal laser scanning microscopy.
Substrate for DNase
Application
Deoxyribonucleic acid methyl green has been used in DNA methyl green bioassay.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
低风险生物材料
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Synthesis, DNA-binding, and Antiviral Activity of Certain Pyrazolo [3, 4-d] pyrimidine Derivatives
El-Bendary ER and Badria FA
Arch. Pharm. (Weinheim), 333(4), 99-103 (2000)
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
Prieto D, et al.
Journal of Visualized Experiments, 315(99) (2015)
Methyl green: A DNA major-groove binding drug
Kim SK and Norden B
Febs Letters, 315(1), 61-64 (1993)
Cytotoxic and DNA interaction activities of extracts from medicinal plants used in Argentina
Mongelli E, et al.
Journal of Ethnopharmacology, 71(1-2), 145-151 (2000)
Simultaneous detection of DAB and methyl green signals on apoptotic nuclei by confocal laser scanning microscopy
Itoh J, et al.
Acta Histochemica Et Cytochemica, 36(4), 367-376 (2003)
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