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Merck
CN

D2554

Dulbecco′s Modified Eagle′s Medium - high glucose

liquid, 10 ×, suitable for cell culture

Synonym(s):

DME, DMEM

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About This Item

UNSPSC Code:
12352207
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sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.
With 4500 mg/L glucose at 1X. Without L-glutamine, folic acid and sodium bicarbonate.
Substitutes pyridoxine hydrochloride for pyridoxal hydrochloride.


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