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About This Item
UNSPSC Code:
12352207
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Preparation Note
Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.
With 4500 mg/L glucose at 1X. Without L-glutamine, folic acid and sodium bicarbonate.
Substitutes pyridoxine hydrochloride for pyridoxal hydrochloride.
Substitutes pyridoxine hydrochloride for pyridoxal hydrochloride.
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