D3065
Diacylglycerol Kinase from Escherichia coli
≥2 units/mg protein
Synonym(s):
sn-1,2-Diacylglycerol kinase, DAGK
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About This Item
specific activity
≥2 units/mg protein
mol wt
13.7 kDa
UniProt accession no.
storage temp.
−70°C
Gene Information
Escherichia coli K12 ... dgkA(948543)
Application
Diacylglycerol Kinase from Escherichia coli has been used in a study to assess the antagonistic regulation of dgkA and plsB genes of phospholipid synthesis by multiple stress responses in Escherichia coli. Diacylglycerol Kinase from Escherichia coli has also been used in a study to identify an alcohol binding site in the first cysteine-rich domain of protein kinase Cdelta.
Biochem/physiol Actions
Specific for the sn-1,2 isomer of DAG. Can be used to measure ceramide production.
Physical form
Supplied as a turbid membrane suspension in 25 mM sodium phosphate buffer, pH 7.0, 20.0% glycerol, 1 mM DTT.
Other Notes
One unit will phosphorylate 1.0 μmol of diacylglycerol per minute at 25°C.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Astrid Wahl et al.
Molecular microbiology, 80(5), 1260-1275 (2011-04-06)
Phospholipid homeostasis of the bacterial membrane is maintained by biochemical regulation of the synthesis enzymes depending on the environment. However, genes encoding phospholipid synthesis enzymes might also be regulated during stress responses, in order for the bacteria to adapt their
Masato Takahashi et al.
Biochemical and biophysical research communications, 423(3), 571-576 (2012-06-15)
The type I diacylglycerol kinase (DGK) isozymes (α, β and γ) contain a shared recoverin homology (RVH) domain, a tandem repeat of Ca2+-binding EF-hand motifs, two cysteine-rich C1 domains, and the catalytic domain. We previously reported that a DGKα mutant
Total chemical synthesis of an integral membrane enzyme: diacylglycerol kinase from Escherichia coli.
Sunanda Lahiri et al.
Angewandte Chemie (International ed. in English), 50(17), 3988-3992 (2011-03-25)
Yasukazu Hozumi et al.
Advances in biological regulation, 52(2), 315-325 (2012-07-12)
Phosphoinositide cycle plays a pivotal role in neuronal signal transduction. In this cycle, diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to yield phosphatidic acid (PA). DG and PA acts as important second messengers that regulate distinct cascade of cellular events. Previous
Maria V Meringer et al.
Plant physiology and biochemistry : PPB, 58, 83-88 (2012-07-13)
We analyzed lipid kinase and lipid phosphatase activities and determined endogenous phytohormone levels by liquid chromatography-tandem mass spectrometry in root and coleoptile tissues following germination of barley (Hordeum vulgare) seeds. The enzymes showing highest activity in aleurone cells were diacylglycerol
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