D3656
Dulbecco′s Modified Eagle′s Medium - high glucose
With 4500 mg/L glucose and L-glutamine, without sodium phosphate and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME, DMEM
form
powder
IVD
for in vitro diagnostic use
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
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Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Preparation Note
Formulated to contain 13.3 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate, 0.109 g/L sodium phosphate monobasic (anhydrous).
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Elena Seiradake et al.
The EMBO journal, 30(21), 4479-4488 (2011-09-29)
Brain wiring depends on cells making highly localized and selective connections through surface protein-protein interactions, including those between NetrinGs and NetrinG ligands (NGLs). The NetrinGs are members of the structurally uncharacterized netrin family. We present a comprehensive crystallographic analysis comprising
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