DNA Sequencing Protocols, 2nd ed.

Major advances have made PCR-based semiautomated fluorescent sequencing the norm. This new edition provides up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. It offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. It offers easy-to-follow methods that will improve the accuracy and quality of DNA sequences obtained by smaller laboratories and help lay the foundation for molecular diagnostics.