D5292
DNA Ladder, Supercoiled
for supercoiled DNA electrophoresis
grade
Molecular Biology
form
liquid
usage
250 uses (25 μg size), 50 uses (10 μg size)
suitability
suitable for electrophoresis (DNA)
shipped in
dry ice
storage temp.
−20°C
General description
Sigma′s DNA Ladder (Supercoiled) contains 11 supercoiled DNA fragments from 2,067 – 16,210 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Other Notes
Contains 11 supercoiled fragments, 2,067–16,210 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.
Sigma′s DNA Ladder (Supercoiled) is provided in a solution of 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Michael J Molloy et al.
Nucleic acids research, 32(16), e129-e129 (2004-09-11)
Within the biopharmaceutical industry, recombinant plasmid DNA is used both as a raw material (e.g. in lentiviral and AAV vector production) as well as an active ingredient (e.g. in DNA vaccines). Consequently, many analytical laboratories are routinely involved with plasmid
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