D6165
Demecolcine
Hybri-Max™, powder, γ-irradiated, suitable for hybridoma
Synonym(s):
N-Deacetyl-N-methylcolchicine
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About This Item
Empirical Formula (Hill Notation):
C21H25NO5
CAS Number:
Molecular Weight:
371.43
Beilstein:
2822892
EC Number:
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
grade
Hybri-Max™
sterility
γ-irradiated
form
powder
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
SMILES string
CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
InChI
1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
InChI key
NNJPGOLRFBJNIW-HNNXBMFYSA-N
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Application
Recommended for use in culture medium at a final concentration of 0.4 μg/ml. Incubate cultures for 4-6 hours at 37 °C. Remove medium containing demecolcine, replace with fresh medium.
Biochem/physiol Actions
Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Preparation Note
Reconstitute 1 mg vial demecolcine with 10 mL of sterile balanced salt solution. After reconstitution, store at 2-8 °C or in frozen aliquots at −20 °C. Repeated freezing and thawing is not recommended.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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BioEssays : news and reviews in molecular, cellular and developmental biology, 34(9), 760-770 (2012-06-19)
Recent studies indicate that mammalian chromosomes contain discrete cis-acting loci that control replication timing, mitotic condensation, and stability of entire chromosomes. Disruption of the large non-coding RNA gene ASAR6 results in late replication, an under-condensed appearance during mitosis, and structural
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Theriogenology, 75(3), 527-541 (2010-11-16)
Treatment of pre-activated oocytes with demecolcine (DEM) has been shown to induce the extrusion of all oocyte chromosomes within the second polar body (PB2). However, induced enucleation (IE) rates are generally low and the competence of these cytoplasts to support
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