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D9683

Col E1 plasmid DNA from Escherichia coli strain C600

buffered aqueous solution

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About This Item

CAS Number:
100209-25-4
UNSPSC Code:
12352200
MDL number:
MFCD00166685
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form

buffered aqueous solution

mol wt

4.4 MDa (approx. 6,600 bp)

storage temp.

−20°C

Biochem/physiol Actions

Comments: Bacterial strains containing the ColE1 plasmid produce colicin E1, a protein that inhibits the growth of other Escherichia coli strains. They are themselves immune to low levels of colicin E1. Immunity can be tested by challenging strains with colicin E1 (Product No. C3026).
Unique Sites: Within the gene for colicin production: Ava I, EcoR I, Sac II, Sca I, Sma I.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, and 1 mM EDTA

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Lot/Batch Number
097H91201
097H9120

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M A Lovett et al.
Proceedings of the National Academy of Sciences of the United States of America, 71(10), 3854-3857 (1974-10-01)
The product of the induced relaxation of supercoiled DNA-protein relaxation complexes of colicinogenic factors E1 (ColE1) and E2 (ColE2) is an open circular DNA molecule with a strand-specific nick. Cleavage of the open circular DNA of each relaxed complex with