DNEP
Deoxyribonuclease I from bovine pancreas
lyophilized powder, Protein ≥70 %, ≥1,500 Kunitz units/mg protein
Synonym(s):
DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-667-0
MDL number:
form
lyophilized powder
specific activity
≥1,500 Kunitz units/mg protein
mol wt
~31 kDa
purified by
chromatography
composition
Protein, ≥70%
packaging
vial of ≥5.0 mg solid
foreign activity
RNase <0.01%
storage temp.
−20°C
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Application
Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Used for the removal of DNA from protein samples.
Physical form
Contains glycine
Analysis Note
Protein determined by biuret.
Other Notes
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Julien Gros et al.
The EMBO journal, 33(6), 621-636 (2014-02-26)
The separation of DNA replication origin licensing and activation in the cell cycle is essential for genome stability across generations in eukaryotic cells. Pre-replicative complexes (pre-RCs) license origins by loading Mcm2-7 complexes in inactive form around DNA. During origin firing
Daniel J Gaffney et al.
PLoS genetics, 8(11), e1003036-e1003036 (2012-11-21)
Nucleosomes are important for gene regulation because their arrangement on the genome can control which proteins bind to DNA. Currently, few human nucleosomes are thought to be consistently positioned across cells; however, this has been difficult to assess due to
Andrew B Stergachis et al.
Science (New York, N.Y.), 342(6164), 1367-1372 (2013-12-18)
Genomes contain both a genetic code specifying amino acids and a regulatory code specifying transcription factor (TF) recognition sequences. We used genomic deoxyribonuclease I footprinting to map nucleotide resolution TF occupancy across the human exome in 81 diverse cell types.
Maria V Rogacheva et al.
The Journal of biological chemistry, 289(9), 5664-5673 (2014-01-10)
Crossing over between homologous chromosomes is initiated in meiotic prophase in most sexually reproducing organisms by the appearance of programmed double strand breaks throughout the genome. In Saccharomyces cerevisiae the double-strand breaks are resected to form three prime single-strand tails
Clément Lafon-Placette et al.
The New phytologist, 197(2), 416-430 (2012-12-21)
DNA methylation is involved in the control of plant development and adaptation to the environment through modifications of chromatin compaction and gene expression. In poplar (Populus trichocarpa), a perennial plant, variations in DNA methylation have been reported between genotypes and
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