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Merck
CN

E0273

pcDNA 1.1/Amp expression vector

lyophilized powder

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About This Item

UNSPSC Code:
12352200
MDL number:
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form

lyophilized powder

storage temp.

−20°C

Biochem/physiol Actions

The MCS contains unique BamH I, EcoR I, EcoR V, Hind III, Not I, Nsi I, Sph I, Xba I, and Xho I restriction sites, and redundant BstX I sites.

Features and Benefits

A 4.8-kb vector for transient, high-level protein expression in mammalian cells. The vector incorporates the strong cytomegalovirus (CMV) enhancer-promoter sequence for high-level expression, SV40 and polyoma origins of replication to facilitate episomal replication, an ampicillin resistance gene for selection in E. coli, and the col E1 origin and supF suppressor tRNA for growth and maintenance in appropriate strains of E. coli.


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