E0379
Enolase from rabbit muscle
ammonium sulfate suspension, ≥15 units/mg protein
Synonym(s):
2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-754-3
MDL number:
form
ammonium sulfate suspension
specific activity
≥15 units/mg protein
foreign activity
lactic dehydrogenase ≤0.1%, pyruvate kinase ≤0.1%
storage temp.
2-8°C
Application
Enolase from rabbit muscle has been used to study its rate-limiting steps and the site of Li+ inhibition.
Biochem/physiol Actions
Enolase is a metalloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis.
Physical form
Crystalline suspension in 2.8 M (NH4)2SO4 containing 0.05 M imidazole and 1 mM MgSO4, pH 7.5
Other Notes
One unit will convert 1.0 μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH 7.4 at 25 °C.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Paola Cappello et al.
Gastroenterology, 144(5), 1098-1106 (2013-01-22)
Pancreatic ductal adenocarcinoma (PDA) is an aggressive tumor, and patients typically present with late-stage disease; rates of 5-year survival after pancreaticoduodenectomy are low. Antibodies against α-enolase (ENO1), a glycolytic enzyme, are detected in more than 60% of patients with PDA
G P Comi et al.
Annals of neurology, 50(2), 202-207 (2001-08-17)
A severe muscle enolase deficiency, with 5% of residual activity, was detected in a 47-year-old man affected with exercise intolerance and myalgias. No rise of serum lactate was observed with the ischemic forearm exercise. Ultrastructural analysis showed focal sarcoplasmic accumulation
Wai Fook Leong et al.
Cellular microbiology, 8(4), 565-580 (2006-03-22)
Insights into the host antiviral strategies as well as viral disease manifestations can be achieved through the elucidation of host- and virus-mediated transcriptional responses. An oligo-based microarray was employed to analyse mRNAs from rhabdomyosarcoma cells infected with the MS/7423/87 strain
Xidao Wang et al.
Neuron, 78(2), 312-324 (2013-04-30)
To what extent dorsal horn interneurons contribute to the modality specific processing of pain and itch messages is not known. Here, we report that loxp/cre-mediated CNS deletion of TR4, a testicular orphan nuclear receptor, results in loss of many excitatory
Hirofumi Ochi et al.
FEBS letters, 528(1-3), 197-202 (2002-09-26)
Hashimoto's encephalopathy (HE) is a rare autoimmune disease associated with Hashimoto's thyroiditis (HT). To identify the HE-related autoantigens, we developed a human brain proteome map using two-dimensional electrophoresis and applied it to the immuno-screening of brain proteins that react with
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