E0526
Endonuclease III from Escherichia coli
≥90% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, buffered aqueous glycerol solution, ≥10,000 units/mg protein
Synonym(s):
Nth protein, Endo III
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About This Item
UNSPSC Code:
12352204
MDL number:
biological source
Escherichia coli
recombinant
expressed in E. coli overproducing strain
assay
≥90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
≥10,000 units/mg protein
mol wt
≥23 kDa (211 amino acids)
technique(s)
activity assay: suitable
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
Escherichia coli K12 ... nth(947122)
Biochem/physiol Actions
Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme. It possesses DNA glycosylase activity with broad substrate specificity for mutated pyrimidine derivatives especially thymine glycol (Tg) but also for 5-hydroxycytosine and 5,6-dihydrothymine. In addition, it has an apurinic/apyrimidinic (AP) lyase activity which cleaves the DNA backbone by β elimination. Endo III protects E. coli cells from radiation damage caused by x-ray and UV irradiation and its activity overlaps the activity of endonuclease VIII. When overexpressed, it can also protect E.coli cells from alkylation defects caused by methylmethanesulphonate.
Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme.
Physical form
Solution in 20 mM Tris, pH 7.4, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% glycerol.
Preparation Note
Purified in an iron bound form that confers the protein a light green color.
Other Notes
One unit will cleave 50% of 0.5 pmol double strand oligonucleotide substrate containing 5,6-dihydrothymine in 15 min. at 30 °C.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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