E1272
Lyo ECM Gel
lyophilized powder, BioReagent, suitable for cell culture
Synonym(s):
Basement membrane extract (BME), EHS matrix, Extracelular matrix, ECM
product line
BioReagent
Quality Level
form
lyophilized powder
concentration
8-12 mg/mL (after reconstitution)
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
General description
ECM gel is a solubilized basement membrane matrix secreted and extracted from EHS mouse sarcoma cells that works as a complex network of proteins and other molecules that surround, support, and give structure to mammalian tissues. Lyo ECM Gel is a novel lyophilized version of the normal ECM gel product providing adjustable research concentrations, enhancing product stability and ease-of-use for many biological applications. The extracellular matrix helps cells attach to, and communicate with, nearby cells. It plays an important role in cell functioning, including growth and movement. Commonly used for: Culturing of invasive cells, induce stem cells proliferation and differentiation, formation of organoids, suitable for the development of cancer cells and xenografts.
Prevention of cancer metastasis must be a major goal of cancer therapy since metastasis is the most insidious and life-threatening aspect of cancer . 3D tumor organoid systems have been developed for cancer cell types. These complex, self-organizing structures are cultured forms of tumor cells embedded and growth supported by ECM GEL.
ECM gel contains laminin as a major component, collagen type IV, heparan sulfate proteoglycan, entactin, and other minor components. ECM gel undergoes thermally activated polymerization when brought to 20-40°C to form a reconstituted basement membrane. The process of gelation is reversible. Addition of collagen type IV to ECM gel increases polymerization, whereas, addition of collagen type I, fibronectin, or heparin, does not. PC12 cells show neurite formation within 2 days when grown on a thin layer of ECM Gel.
Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV
Prevention of cancer metastasis must be a major goal of cancer therapy since metastasis is the most insidious and life-threatening aspect of cancer . 3D tumor organoid systems have been developed for cancer cell types. These complex, self-organizing structures are cultured forms of tumor cells embedded and growth supported by ECM GEL.
ECM gel contains laminin as a major component, collagen type IV, heparan sulfate proteoglycan, entactin, and other minor components. ECM gel undergoes thermally activated polymerization when brought to 20-40°C to form a reconstituted basement membrane. The process of gelation is reversible. Addition of collagen type IV to ECM gel increases polymerization, whereas, addition of collagen type I, fibronectin, or heparin, does not. PC12 cells show neurite formation within 2 days when grown on a thin layer of ECM Gel.
Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV
Preparation Note
Lyo ECM Gel is recomended to be reconstituted with 10 ml of water (#W3500) for a concentration as described in COA specifications (8-12 mg/ml). Lyo ECM Gel can be storage at room temperature for up to 4 months. The reconstituted liquid Lyo ECM Gel can be keep upto 7 days at 4oC. During work is recomended to keep the vial in ice or in a temperature under 10 oC
Disclaimer
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Patrick Mehlen et al.
Nature reviews. Cancer, 6(6), 449-458 (2006-05-26)
The metastatic process is highly inefficient--very few of the many cells that migrate from the primary tumour successfully colonize distant sites. One proposed mechanism to explain this inefficiency is provided by the cancer stem cell model, which hypothesizes that micrometastases
D J Carey et al.
The Journal of cell biology, 102(6), 2254-2263 (1986-06-01)
Exposing rat Schwann cells co-cultured with nerve cells to a reconstituted basement membrane induced the formation of myelin segments by Schwann cells. This occurred in a serum-free culture medium in which, in the absence of this matrix, Schwann cells proliferate
Articles
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
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