Monoclonal Anti-eIF5A antibody produced in mouse

Monoclonal Anti-eIF5A antibody produced in mouse is suitable for immunocytochemistry and western blot analysis at a working concentration of 1-2μg/mL using 3T3 total cell extract.

EIF5A (Eukaryotic translation initiation factor 5A-1) gene encodes a mRNA-binding protein involved in translation elongation. It also has an important role in mRNA turnover, mRNA decay, actin dynamics and cell cycle progression. It is also implicated in cell proliferation, synthesis of proteins involved in cell cycle control, and transport of viral mRNAs from nucleus to cytoplasm in association with Rev HIV-I protein. Higher eIF5A protein expression levels are present in lung adenocarcinomas tumors, suggesting eIF5A as a prognostic marker in lung carcinomas. It is a highly conserved protein that undergoes a unique and essential post-translational modification dependent on the polyamine spermidine, called hypusination. Reduction of eIF5A content is implicated in brain aging.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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