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E2412

Sigma-Aldrich

Pullulanase microbial

Synonym(s):

Pullulanase microbial, Promozyme® D2

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About This Item

CAS Number:
9075-68-7
EC Number:
3.2.1.41 (BRENDA, IUBMB)
MDL number:
MFCD00081941
UNSPSC Code:
12352204
NACRES:
NA.54

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recombinant

expressed in Bacillus subtilis

form

aqueous solution

specific activity

≥1000 NPUN/g

storage temp.

2-8°C

Related Categories

Application

Pullulanase is a glucanase that degrades pullulan. It is commonly used for starch-debranching as well as to study polysaccharide utilization.

Biochem/physiol Actions

Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

A product of Novozymes Corp.
Novozym is a registered trademark of Novozymes A/S
Promozyme is a trademark of Novozymes Corp.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000489804
0000489804
0000373134
0000305950
0000305951

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K A Smith et al.
Journal of bacteriology, 171(4), 2116-2123 (1989-04-01)
We have cloned a pullulanase gene from Bacteroides thetaiotaomicron. The pullulanase expressed from this clone in Escherichia coli was cell associated and soluble and had a molecular mass of 72 kilodaltons by gel filtration. Maxicell analysis of proteins coded by
Nasima Chorfa et al.
Polymers, 14(10) (2022-05-29)
In this work, a valorization of the starch stemming from downgraded potatoes was approached through the preparation of starch nanoparticles using different physical methods, namely liquid and supercritical carbon dioxide, high energy ball milling (HEBM), and ultrasonication on the one
Kornelia T Kaczmarska et al.
Journal of agricultural and food chemistry, 65(46), 10064-10073 (2017-10-24)
This study investigated the effect of germination and fermentation on the composition of carbohydrates in Australian sweet lupin. Specifically, the amount of sugars (sucrose, fructose, and glucose), starch, oligosaccharides (verbascose, stachyose, and raffinose), and dietary fiber were measured in germinated
Grzegorz Kłosowski et al.
Journal of bioscience and bioengineering, 109(5), 466-471 (2010-03-30)
The aim of the research was to assess the possibility of the fermentation productivity rising through the increase in corn mashes extract from 16-17 to 20-21 degrees Balling, yet keeping a 3-day fermentation period. The second goal was to obtain
Guodong Liu et al.
Carbohydrate polymers, 98(1), 995-1001 (2013-08-31)
This study aims to investigate the physicochemical and extended-release properties of amorphous debranched starch (ADBS) that is a linear short chain amylose derived from pullulanase enzymatic modification. The results show the completely amorphous ADBS was soluble in cold water, which
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