GenElute^™ RNA/DNA/Protein Plus Purification Kit

The GenElute RNA/DNA/Protein Plus Purification Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. This kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). DNA and RNA purification is based on spin column chromatography. The process involves first lysing the cells or tissue of interest with the provided Lysis Solution. The DNA is then captured and purified on a DNA Purification Column. Ethanol is then added to the flowthrough of the DNA purification step, and the solution is loaded onto a RNA/Protein Purification Column. The resin in the column binds nucleic acids in a manner that depends on ionic concentrations, thus only the RNA including microRNAs will bind to the column while the proteins are removed in the flowthrough. Next, the bound RNA is washed with the provided RNA Wash Solution to remove impurities, and the purified RNA is eluted with the RNA Elution Solution.

The proteins that are present from the RNA binding flowthrough can now be loaded directly onto an SDS-PAGE gel for visual analysis. Alternatively, the protein samples can be further purified using the same RNA/Protein Purification Column that was used for purifying the RNA. After the RNA has been eluted from the column, the flowthrough is then pH adjusted and loaded back onto the column in order to bind the proteins that are present. The bound proteins are washed with the provided wash buffer, and are then eluted such that they can be used in downstream applications.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Kit Components and Storage Conditions

This product is a kit component of E5163-1KT, which is shipped in two parts: 1) Cat. No. E5164-1KT, includes reagents shipped at room temperature, and 2) Cat. No. 23506NB, Protein Loading Dye, is shipped in dry ice.

GenElute is a trademark of Sigma-Aldrich Co. LLC