E6754
Epoxy-activated−Sepharose™ 6B
lyophilized powder
Synonym(s):
Epoxy-activated-Agarose
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About This Item
form
lyophilized powder
Quality Segment
extent of labeling
19-40 μmol per mL
technique(s)
affinity chromatography: suitable
matrix
Sepharose 6B
matrix active group
epoxy
matrix attachment
through epoxy to hydroxyl
matrix spacer
12 atoms
swelling
1 g swells to 3.0-5.0 mL
particle size
45—165 μm
storage temp.
2-8°C
General description
when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.
Application
Epoxy-activated-Sepharose™ is used in affinity chromatography, protein chromatography, and activated/functionalized matrices. Epoxy-activated-Sepharose™ has been used to obtain a monoclonal antibody against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B as well as to study B meningococcal disease.
Physical form
Lyophilized powder
Other Notes
Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.
Legal Information
Sepharose is a trademark of Cytiva
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M G Marcu et al.
Journal of the National Cancer Institute, 92(3), 242-248 (2000-02-03)
Heat shock protein 90 (Hsp90) interacts with and stabilizes several oncogenic protein kinases (e.g., p185(erbB2), p60(v-src), and Raf-1) and is required for the stability and dominant-negative function of mutated p53 protein. Two unrelated antibiotics, geldanamycin and radicicol, bind specifically to
Kasim Abass Askar et al.
Applied biochemistry and biotechnology, 165(1), 336-346 (2011-04-19)
The purpose of this study was to develop a protocol for the purification of acetylcholinesterase (AChE, acetylcholine acetylhydrolase, E.C.3.1.1.7) enzyme and to extend a purification method for further enzyme characterization. A further aim was to study whether the edrophonium's pharmacologic
Adam Frankel et al.
RNA (New York, N.Y.), 9(7), 780-786 (2003-06-18)
The universal genetic code links the 20 naturally occurring amino acids to the 61 sense codons. Previously, the UAG amber stop codon (a nonsense codon) has been used as a blank in the code to insert natural and unnatural amino
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E6754-5G | 04061833604632 |