E7033
pFLAG-CMV-2 Expression Vector
shuttle vector for intracellular transient expression of N-terminal Met-FLAG
Product Name
pFLAG-CMV-2 Expression Vector, shuttle vector for intracellular transient expression of N-terminal Met-FLAG
tag
FLAG® tagged
grade
Molecular Biology
form
buffered aqueous solution
quality
shuttle vector for intracellular transient expression of N-terminal Met-FLAG
peptide tag location
N-terminal
shipped in
dry ice
storage temp.
−20°C
Legal Information
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Biochem/physiol Actions
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.
General description
The pFLAG-CMV™-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
Other Notes
- pFLAG-CMV™-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
- pFLAG-CMV™-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Storage Class
10 - Combustible liquids
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Li Yang et al.
Cancer research, 67(12), 5587-5593 (2007-06-19)
Evidence indicates that the induction of cyclooxygenase-2 (COX-2) and high prostaglandin E2 (PGE2) levels contribute to the pathogenesis of non-small-cell lung cancer (NSCLC). In addition to overproduction by COX-2, PGE2 concentrations also depend upon the levels of the PGE2 catabolic
Jing Ouyang et al.
Blood, 117(16), 4315-4322 (2011-02-09)
Posttransplant lymphoproliferative disorders (PTLDs) are potentially fatal, EBV-driven B-cell malignancies that develop in immunocompromised solid organ or hematopoietic stem cell recipients. In PTLD, the expression of EBV proteins, including latent membrane protein 1 (LMP1) and LMP2A, viral immune evasion strategies
Michiko Tanaka et al.
Virology journal, 5, 125-125 (2008-10-23)
The herpes simplex virus 1 (HSV-1) UL7 gene is highly conserved among herpesviridae. Since the construction of recombinant HSV-1 with a mutation in the UL7 gene has not been reported, the involvement of HSV-1 UL7 in viral replication has been
Zhujun Zhang et al.
Cancer epidemiology, 33(2), 130-136 (2009-08-15)
IFN regulatory factor 4-binding protein (IBP) is a novel type of activator of Rho GTPases. It has been linked with differentiation and apoptosis of lymphocytes, but its function in oncogenesis remains unclear. Here we studied the expression of endogenous IBP
Kohsuke Tsuchiya et al.
Journal of immunology (Baltimore, Md. : 1950), 185(2), 1186-1195 (2010-06-23)
Listeria monocytogenes invades the cytoplasm of macrophages and induces the activation of caspase-1 and the subsequent maturation of IL-1beta and IL-18. Although apoptosis-associated speck-like protein containing a caspase-activating and recruitment domain (ASC), an adaptor protein of nucleotide-binding oligomerization domain (Nod)-like
Related Content
Instructions
pFLAG-CMV-1 and pFLAG-CMV-2 Vector Maps
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service