E7403
Anti-S1P1, C-Terminal antibody produced in rabbit
~1 mg/mL, fractionated antiserum, buffered aqueous solution
Synonym(s):
Anti-EDG-1, Anti-Endothelial Cell Differentiation Gene-1
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~44 kDa by SDS-PAGE
species reactivity
human
concentration
~1 mg/mL
General description
Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein encoded by the S1PR1 gene in humans. It is a bioactive sphingolipid present at high concentrations in plasma and lymphatic fluid. S1P plays a critical role in regulating lymphocyte trafficking mainly through sphingosine-1-phosphate receptor 1 (S1PR1). It is a blood-borne lysosphingolipid that acts to promote endothelial cell (EC) barrier function. In plasma, it is associated with both high density lipoproteins (HDL) and albumin.
Immunogen
synthetic peptide (approximately 2.5 kDa) derived from the C-terminal region of the EDG-1/S1P1 receptor.
Application
Anti-S1P1, C-Terminal antibody produced in rabbit is suitable for western blotting at a working concentration of 5 to 10 μg/ml using RH7777 cells transfected with full-length EDG-1 receptor.
Biochem/physiol Actions
Sphingosine-1-phosphate (SPP) activates the heterotrimeric guanine nucleotide binding protein (G protein)-coupled orphan receptor EDG-1. EDG-1 is a high-affinity receptor for the bioactive lipid mediator SPP. It binds to SPP with high affinity and specificity. Overexpression of EDG-1 induces exaggerated cell-cell aggregation, enhances expression of cadherins and formation of well-developed adherens junctions. It is essential for lymphocyte egress from secondary lymphoid organs and acts as a validated drug target for the treatment of autoimmune diseases. Activated EDG-1 induces an immediate increase in intracellular calcium and is able to increase S1P1 phosphorylation.
Physical form
Supplied in phosphate buffered saline, pH 7.3, containing 0.08% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
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