E8033
pFLAG-MAC™ Expression Vector
Bacterial vector for cytoplasmic expression of N-terminal Met-FLAG fusion proteins
tag
FLAG® tagged
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
General description
The pFLAG-MAC™ Expression Vector is a 5.0 kb E. coli expression vector used for cloning and cytoplasmic expression of properly inserted open reading frame as an N-terminal Met-FLAG® fusion protein. The Met-FLAG fusion protein may be detected using Monoclonal ANTI-FLAG M2 (F3165), Monoclonal ANTI-FLAG M5 (F4042), and can be purified using the ANTI-FLAG M2 Affinity Gel (A2220).
The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein. The FLAG-BAP fusion protein may be detected using Monoclonal ANTI-FLAG M1 (F3040), or Monoclonal ANTI-FLAG M2 (F3165), or purified using ANTI-FLAG M1 Agarose Affinity Gel (A4596), or ANTI-FLAG M2 Affinity Gel (A2220).
Vector Maps and Sequences
The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein. The FLAG-BAP fusion protein may be detected using Monoclonal ANTI-FLAG M1 (F3040), or Monoclonal ANTI-FLAG M2 (F3165), or purified using ANTI-FLAG M1 Agarose Affinity Gel (A4596), or ANTI-FLAG M2 Affinity Gel (A2220).
Vector Maps and Sequences
Other Notes
- pFLAG-MAC™ Expression Vector 10 μg (E5644) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-ATS-BAP Control Plasmid 1 μg (C9079) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-MAC is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
Regulatory Information
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