E8408
pFLAG-CTC™ Expression Vector
Bacterial vector for cytoplasmic expression of C-terminal FLAG fusion proteins
Product Name
pFLAG-CTC™ Expression Vector, Bacterial vector for cytoplasmic expression of C-terminal FLAG fusion proteins
tag
FLAG® tagged
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
Legal Information
pFLAG-CTS is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CTC is a trademark of Sigma-Aldrich Co. LLC
Biochem/physiol Actions
The promoter-regulatory region of the strong tac promoter (a hybrid of the trp and lac promoters from E.coli) drives transcription of ORF-FLAG fusion constructs. Control of transcription is regulated by the presence of the lacO sequences and inclusion of the lac repressor gene (lacI) on the plasmid.
General description
The pFLAG-CTC™ Expression Vector is a 5.3 kb E. coli expression vector used for cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein. The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products.
C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb vE. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb vE. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
Other Notes
- pFLAG-CTC™ Expression Vector 10 μg (E5394) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-CTS™-BAP Control Plasmid 1 μg (P7707) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Storage Class
10 - Combustible liquids
Regulatory Information
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The tac promoter: a functional hybrid derived from the trp and lac promoters.
DeBoer, H. A., et al.
Proceedings of the National Academy of Sciences of the USA, 80, 21-25 (1993)
Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing.
D R Russell et al.
Gene, 20(2), 231-243 (1982-12-01)
Koichiro Komiya et al.
Arthritis research & therapy, 7(6), R1158-R1173 (2005-11-10)
ADAMs (a disintegrin and metalloproteinases) comprise a new gene family of metalloproteinases, and may play roles in cell-cell interaction, cell migration, signal transduction, shedding of membrane-anchored proteins and degradation of extracellular matrix. We screened the mRNA expression of 10 different
Tsuyoshi Miki et al.
Journal of bacteriology, 191(22), 6843-6854 (2009-09-22)
The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded
Johanna M Schwingel et al.
Glycobiology, 18(6), 447-455 (2008-03-14)
Moraxella catarrhalis express three predominant forms of lipooligosaccharide (LOS) molecules on the bacterial surface. These major glycolipids contain specific carbohydrate epitopes that distinguish each glycoform into serotype A, B, or C LOS. All three serotypes, however, share a common glucose
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Bacterial Expression Vectors: tac Promoter System
pFLAG-CTC and pFLAG-CTS
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