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Merck
CN

E8658

pFLAG-Myc-CMV-19 Expression Vector

Shuttle vector for transient expression of secreted dual tagged N-terminal FLAG and C-terminal c-myc fusion proteins

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About This Item

UNSPSC Code:
12352200
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tag

FLAG® tagged, c-Myc tagged (C-terminal)

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

Application

The pFLAG-Myc-CMV-19 Expression Vector (4.7 kb) is used to establish transient expression of secreted, dual-tagged N-terminal FLAG® and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes the FLAG® epitope (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively.

Expression vector and control vector sequences can be found here.

Other Notes

Browse additional application references in our FLAG® Literature portal.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC


Storage Class

10 - Combustible liquids

Regulatory Information

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Instructions


A M Campbell et al.
The Journal of biological chemistry, 267(13), 9321-9325 (1992-05-05)
The sarcoplasmic/endoplasmic reticulum slow-twitch or cardiac Ca(2+)-ATPase (SERCA2) is expressed as two forms (SERCA2a and SERCA2b) which vary at their extreme carboxyl termini. SERCA2a and SERCA2b are derived from alternatively spliced primary transcripts of the same gene. These two alternative
S Andersson et al.
The Journal of biological chemistry, 264(14), 8222-8229 (1989-05-15)
The conversion of cholesterol into bile acids in the liver represents the major catabolic pathway for the removal of cholesterol from the body. In this complex biosynthetic pathway, at least 10 enzymes modify both the ring structure and side chain