The pFLAG-Myc-CMV^™-22 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of dual-tagged N-terminal FLAG^® and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes the FLAG epitope (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively.

pFLAG-Myc-CMV-22 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.

The pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

The pFLAG-Myc-CMV^™-20 Expression Vector is suitable for stable cytoplasmic expression of C-terminal c-myc N-terminal Met-FLAG^® fusion proteins in mammalian cells.

pFLAG-Myc-CMV^™-22 Expression Vector 20 μg (E5901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pFLAG-CMV^™-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG^® and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (Neo-r) confers resistance to aminoglycosides such as G418 allowing for selection of stable transfectants.

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

pFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC