E9033
pFLAG-Myc-CMV™-22 Expression Vector
Shuttle vector for transient or stable intracellular expression of dual tagged N-terminal FLAG and C-terminal c-myc fusion proteins
tag
FLAG® tagged
c-Myc tagged (C-terminal)
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
General description
The pFLAG-Myc-CMV™-22 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of dual-tagged N-terminal FLAG® and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes the FLAG epitope (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively.
pFLAG-Myc-CMV-22 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
The pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
pFLAG-Myc-CMV-22 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
The pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
Application
The pFLAG-Myc-CMV™-20 Expression Vector is suitable for stable cytoplasmic expression of C-terminal c-myc N-terminal Met-FLAG® fusion proteins in mammalian cells.
Biochem/physiol Actions
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG® and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (Neo-r) confers resistance to aminoglycosides such as G418 allowing for selection of stable transfectants.
Other Notes
pFLAG-Myc-CMV™-22 Expression Vector 20 μg (E5901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pFLAG-CMV™-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pFLAG-CMV™-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
pFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
Regulatory Information
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