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Merck
CN

E9652

Sigma-Aldrich

EphB4/Fc Chimera from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):

Htk, Mdk2, Myk1, Tyro11

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About This Item

MDL number:
MFCD01867528
UNSPSC Code:
51111800

Key Documents

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biological source

mouse

recombinant

expressed in NSO cells

Assay

>95% (SDS-PAGE)

form

lyophilized powder

mol wt

monomer calculated mol wt 85.2 kDa
 ~110 kDa by SDS-PAGE (reducing)

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

ligand binding assay: suitable

impurities

endotoxin, tested

UniProt accession no.

P54761

storage temp.

−20°C

Gene Information

mouse ... Ephb4(13846)

General description

EPHB4 (Ephb1 Eph receptor B4) belongs to EPHB receptor family, which is the biggest family of transmembrane tyrosine kinase receptors. Mammals contain five EPHB receptors, namely, EPHB1-EPHB4 and EPHB6. Their ligands are transmembrane in nature and are called ephrinB1, -B2 and -B3.

Biochem/physiol Actions

EPHB4 is a member of the Eph family of receptor tyrosine kinases, which play a role in regulating cell adhesion and cell movement during embryonic development. Furthermore, because tissue disorganization and abnormal cell adhesion, movement, and survival characterize the more advanced stages of cancer, the inappropriate functioning of Eph receptor could play a role in malignancy. Furthermore, EPHB4 has been shown to be the major essential interaction partner of Ephrin B2 in angiogenesis.
Member of Eph receptor tyrosine kinase family shown to bind ephrin-B1 and ephrin-B2; involved in pattern formation and morphogenesis and arterial/venous demarcation of the developing vasculature. EphB4 is also involved in hematopoiesis.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activity is measured by its ability to bind recombinant mouse ephrin-B2/Fc in an ELISA assay.

Other Notes

Extracellular domain of mouse EphB4 (amino acids 1-539) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
071K0210

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A C Andres et al.
Oncogene, 9(5), 1461-1467 (1994-05-01)
To investigate the involvement of protein tyrosine kinases (PTKs) in the growth control of mammary epithelial cells, we have used PCR based cloning to identify PTKs expressed in a mouse mammary epithelial cell line. This approach led to the isolation
S R Kumar et al.
British journal of cancer, 96(7), 1083-1091 (2007-03-14)
EphB4 is a member of the largest family of transmembrane receptor tyrosine kinases and plays critical roles in axonal pathfinding and blood vessel maturation. We wanted to determine the biological role of EphB4 in ovarian cancer. We studied the expression
Vincent Cibert-Goton et al.
PloS one, 8(1), e53673-e53673 (2013-01-24)
EphB receptors tyrosine kinases and ephrinB ligands were first identified as guidance molecules involved in the establishment of topographical mapping and connectivity in the nervous system during development. Later in development and into adulthood their primary role would switch from

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